Western Blot:Article Title: The HGF-MET axis coordinates liver cancer metabolism and autophagy for chemotherapeutic resistance
Article Snippet: .. Antibodies were obtained from the indicated sources: anti-MET (ThermoFisher, 700261, for WB; Proteintech Group, 25869-1-AP, for immunoprecipitation; Alphamab Co. Ltd, 5D5, for therapy), anti-p-MET Y1003 (Cell Signaling Technology, 3135), anti-p-MET Y1234/1235 (Cell Signaling Technology, 3077, for WB and immunohistochemistry [IHC]), anti-p-MET Y1349 (Cell Signaling Technology, 3133), anti-p-MET Y1356 (Abcam, ab73992), anti-p-MET Y1365 (Santa Cruz Biotechnology, sc-377548), anti-p-tyrosine (Cell Signaling Technology, 8954, for WB and IP), anti-PDHA1/PDH-E1α (Santa Cruz Biotechnology, sc-377092, for WB and IP), anti-DLAT/PDC-E2 (Santa Cruz Biotechnology, sc-271534, for WB and IP), anti-PDHX/E3BP (Santa Cruz Biotechnology, sc-377255, for WB and IP), anti-GLS/GLS1 (Abcam, ab93434, for WB and IP), anti-GLS2 (Abcam, ab150474), anti-GLUL/GS (Abcam, ab64613), anti-PKM/PKM2 (Santa Cruz Biotechnology, sc-365684), anti-LDHA (Santa Cruz Biotechnology, sc-137243), anti-SLC2A1/GLUT1 (Proteintech Group, 21829-1-AP), anti-SLC2A2/GLUT2 (Proteintech Group, 20436-1-AP), anti-SLC2A3/GLUT3 (Proteintech Group, 20403-1-AP), anti-SLC2A4/GLUT4 (Abgent, AP20792a), anti-SLC7A5/LAT1 (Proteintech Group, 13752–1-AP), anti-SLC7A8/LAT2 (Santa Cruz Biotechnology, sc-293242), anti-SLC8A1/SNAT1 (Proteintech Group, 12039-1-AP), anti-SLC8A3/SNAT3 (Proteintech Group, 14315-1-AP), anti-SLC1A5/ASCT2 (Abcam, ab187692), anti-MTOR (Cell Signaling Technology, 9862), Autophagy Antibody Sampler Kit (anti-BECN1, anti-ATG7, anti-ATG5, anti-ATG16L1) (Cell Signaling Technology, 4445), anti-LC3B (Novus, NB100-2220), anti-SQSTM1 (MBL, PM045, for WB and IP; Santa Cruz Biotechnology, sc-28359, for IHC), anti-ATG13 (ABclonal, A0690), anti-ULK1 (Abcam, ab128859), anti-PIK3C3/Vps34 (Abcam, ab124905), anti-UVRAG (Abcam, ab174550), anti-RB1CC1 (Proteintech Group, 17250-1-AP), anti-RUBCN/rubicon (Proteintech Group, 21444-1-AP), anti-RPTOR/raptor (Proteintech Group, 20984-1-AP), anti-Flag (Sigma, F1804), anti-GFP (Santa Cruz Biotechnology, sc-81045), anti-ACTB (Proteintech Group, HRP-60008), anti-rabbit IgG (HRP conjugated; GeneTex, GTX221666-01), and anti-mouse IgG (HRP conjugated; GeneTex, GTX221667-01). ..
Immunoprecipitation:Article Title: The HGF-MET axis coordinates liver cancer metabolism and autophagy for chemotherapeutic resistance
Article Snippet: .. Antibodies were obtained from the indicated sources: anti-MET (ThermoFisher, 700261, for WB; Proteintech Group, 25869-1-AP, for immunoprecipitation; Alphamab Co. Ltd, 5D5, for therapy), anti-p-MET Y1003 (Cell Signaling Technology, 3135), anti-p-MET Y1234/1235 (Cell Signaling Technology, 3077, for WB and immunohistochemistry [IHC]), anti-p-MET Y1349 (Cell Signaling Technology, 3133), anti-p-MET Y1356 (Abcam, ab73992), anti-p-MET Y1365 (Santa Cruz Biotechnology, sc-377548), anti-p-tyrosine (Cell Signaling Technology, 8954, for WB and IP), anti-PDHA1/PDH-E1α (Santa Cruz Biotechnology, sc-377092, for WB and IP), anti-DLAT/PDC-E2 (Santa Cruz Biotechnology, sc-271534, for WB and IP), anti-PDHX/E3BP (Santa Cruz Biotechnology, sc-377255, for WB and IP), anti-GLS/GLS1 (Abcam, ab93434, for WB and IP), anti-GLS2 (Abcam, ab150474), anti-GLUL/GS (Abcam, ab64613), anti-PKM/PKM2 (Santa Cruz Biotechnology, sc-365684), anti-LDHA (Santa Cruz Biotechnology, sc-137243), anti-SLC2A1/GLUT1 (Proteintech Group, 21829-1-AP), anti-SLC2A2/GLUT2 (Proteintech Group, 20436-1-AP), anti-SLC2A3/GLUT3 (Proteintech Group, 20403-1-AP), anti-SLC2A4/GLUT4 (Abgent, AP20792a), anti-SLC7A5/LAT1 (Proteintech Group, 13752–1-AP), anti-SLC7A8/LAT2 (Santa Cruz Biotechnology, sc-293242), anti-SLC8A1/SNAT1 (Proteintech Group, 12039-1-AP), anti-SLC8A3/SNAT3 (Proteintech Group, 14315-1-AP), anti-SLC1A5/ASCT2 (Abcam, ab187692), anti-MTOR (Cell Signaling Technology, 9862), Autophagy Antibody Sampler Kit (anti-BECN1, anti-ATG7, anti-ATG5, anti-ATG16L1) (Cell Signaling Technology, 4445), anti-LC3B (Novus, NB100-2220), anti-SQSTM1 (MBL, PM045, for WB and IP; Santa Cruz Biotechnology, sc-28359, for IHC), anti-ATG13 (ABclonal, A0690), anti-ULK1 (Abcam, ab128859), anti-PIK3C3/Vps34 (Abcam, ab124905), anti-UVRAG (Abcam, ab174550), anti-RB1CC1 (Proteintech Group, 17250-1-AP), anti-RUBCN/rubicon (Proteintech Group, 21444-1-AP), anti-RPTOR/raptor (Proteintech Group, 20984-1-AP), anti-Flag (Sigma, F1804), anti-GFP (Santa Cruz Biotechnology, sc-81045), anti-ACTB (Proteintech Group, HRP-60008), anti-rabbit IgG (HRP conjugated; GeneTex, GTX221666-01), and anti-mouse IgG (HRP conjugated; GeneTex, GTX221667-01). ..
Immunohistochemistry:Article Title: The HGF-MET axis coordinates liver cancer metabolism and autophagy for chemotherapeutic resistance
Article Snippet: .. Antibodies were obtained from the indicated sources: anti-MET (ThermoFisher, 700261, for WB; Proteintech Group, 25869-1-AP, for immunoprecipitation; Alphamab Co. Ltd, 5D5, for therapy), anti-p-MET Y1003 (Cell Signaling Technology, 3135), anti-p-MET Y1234/1235 (Cell Signaling Technology, 3077, for WB and immunohistochemistry [IHC]), anti-p-MET Y1349 (Cell Signaling Technology, 3133), anti-p-MET Y1356 (Abcam, ab73992), anti-p-MET Y1365 (Santa Cruz Biotechnology, sc-377548), anti-p-tyrosine (Cell Signaling Technology, 8954, for WB and IP), anti-PDHA1/PDH-E1α (Santa Cruz Biotechnology, sc-377092, for WB and IP), anti-DLAT/PDC-E2 (Santa Cruz Biotechnology, sc-271534, for WB and IP), anti-PDHX/E3BP (Santa Cruz Biotechnology, sc-377255, for WB and IP), anti-GLS/GLS1 (Abcam, ab93434, for WB and IP), anti-GLS2 (Abcam, ab150474), anti-GLUL/GS (Abcam, ab64613), anti-PKM/PKM2 (Santa Cruz Biotechnology, sc-365684), anti-LDHA (Santa Cruz Biotechnology, sc-137243), anti-SLC2A1/GLUT1 (Proteintech Group, 21829-1-AP), anti-SLC2A2/GLUT2 (Proteintech Group, 20436-1-AP), anti-SLC2A3/GLUT3 (Proteintech Group, 20403-1-AP), anti-SLC2A4/GLUT4 (Abgent, AP20792a), anti-SLC7A5/LAT1 (Proteintech Group, 13752–1-AP), anti-SLC7A8/LAT2 (Santa Cruz Biotechnology, sc-293242), anti-SLC8A1/SNAT1 (Proteintech Group, 12039-1-AP), anti-SLC8A3/SNAT3 (Proteintech Group, 14315-1-AP), anti-SLC1A5/ASCT2 (Abcam, ab187692), anti-MTOR (Cell Signaling Technology, 9862), Autophagy Antibody Sampler Kit (anti-BECN1, anti-ATG7, anti-ATG5, anti-ATG16L1) (Cell Signaling Technology, 4445), anti-LC3B (Novus, NB100-2220), anti-SQSTM1 (MBL, PM045, for WB and IP; Santa Cruz Biotechnology, sc-28359, for IHC), anti-ATG13 (ABclonal, A0690), anti-ULK1 (Abcam, ab128859), anti-PIK3C3/Vps34 (Abcam, ab124905), anti-UVRAG (Abcam, ab174550), anti-RB1CC1 (Proteintech Group, 17250-1-AP), anti-RUBCN/rubicon (Proteintech Group, 21444-1-AP), anti-RPTOR/raptor (Proteintech Group, 20984-1-AP), anti-Flag (Sigma, F1804), anti-GFP (Santa Cruz Biotechnology, sc-81045), anti-ACTB (Proteintech Group, HRP-60008), anti-rabbit IgG (HRP conjugated; GeneTex, GTX221666-01), and anti-mouse IgG (HRP conjugated; GeneTex, GTX221667-01). ..
Incubation:Article Title: Identification of Novel Gene Signature Predicting Lymph Node Metastasis in Papillary Thyroid Cancer via Bioinformatics Analysis and in vitro Validation
Article Snippet: .. Tissue wax samples of three groups namely normal, non-LNM and LNM including 12 tissue samples respectively were heated in citrate buffer for 15 min and rinsed using PBS after soaking in xylene for 40 min and in anhydrous ethanol for 20 min. Then the sections were incubated with primary antibodies including anti-MET (Proteintech, 25869-1-AP, PBS 1:500 dilution), anti-FN1 (Proteintech, 15613-1-AP, PBS1:300 dilution), anti-MPZL2 (Proteintech, 11787-1-AP, PBS 1:200 dilution), anti-PDLIM4 (ThermoFisher, bs-6093R, PBS 1:200 dilution), anti-CLDN10 (ThermoFisher, bs-13739R, PBS 1:200 dilution), and anti-COL8A2 (Affinity Biosciences, DF8903, PBS 1:100 dilution) at 4 °C overnight and goat anti-mouse/rabbit IgG polymer antibody (Immunoway, RS0011, PBS 1:100 dilution) was then added and incubated for 1 h at room temperature after three times washing. ..
Polymer:Article Title: Identification of Novel Gene Signature Predicting Lymph Node Metastasis in Papillary Thyroid Cancer via Bioinformatics Analysis and in vitro Validation
Article Snippet: .. Tissue wax samples of three groups namely normal, non-LNM and LNM including 12 tissue samples respectively were heated in citrate buffer for 15 min and rinsed using PBS after soaking in xylene for 40 min and in anhydrous ethanol for 20 min. Then the sections were incubated with primary antibodies including anti-MET (Proteintech, 25869-1-AP, PBS 1:500 dilution), anti-FN1 (Proteintech, 15613-1-AP, PBS1:300 dilution), anti-MPZL2 (Proteintech, 11787-1-AP, PBS 1:200 dilution), anti-PDLIM4 (ThermoFisher, bs-6093R, PBS 1:200 dilution), anti-CLDN10 (ThermoFisher, bs-13739R, PBS 1:200 dilution), and anti-COL8A2 (Affinity Biosciences, DF8903, PBS 1:100 dilution) at 4 °C overnight and goat anti-mouse/rabbit IgG polymer antibody (Immunoway, RS0011, PBS 1:100 dilution) was then added and incubated for 1 h at room temperature after three times washing. ..
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